EPO 抗体 (AA 28-162) (CF®647)
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- 抗原 See all EPO 抗体
- EPO (Erythropoietin (EPO))
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抗原表位
- AA 28-162
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EPO antibody is conjugated to CF®647
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-Erythropoietin (EPO/1367), CF647 Conjugate
- 产品特性
- This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)
- 克隆位点
- EPO-1367
- 亚型
- IgG
- Top Product
- Discover our top product EPO Primary Antibody
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- 应用备注
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Immunohistology (formalin) 4-8 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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HepG2 Cells. Heart or Kidney.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- EPO (Erythropoietin (EPO))
- 别名
- Erythropoietin (EPO 产品)
- 别名
- EPO antibody, EP antibody, MVCD2 antibody, erythropoietin antibody, erythropoietin S homeolog antibody, erythropoietin a antibody, EPO antibody, epo antibody, epo.S antibody, Epo antibody, epoa antibody
- 物质类
- Hormone
- 背景
- EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2
- 分子量
- 37 kDa
- 基因ID
- 2056, 2303
- UniProt
- P01588
- 途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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