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Complement C4d 抗体

C4d 适用: 人 IF, ELISA, Coat, IHC (f) 宿主: 小鼠 Monoclonal C4D204 unconjugated
产品编号 ABIN6173841
发货至: 中国
  • 抗原 See all Complement C4d (C4d) products
    Complement C4d (C4d) (Complement Component C4d (C4d))
    适用
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    宿主
    • 25
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    小鼠
    克隆类型
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    单克隆
    标记
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    This Complement C4d antibody is un-conjugated
    应用范围
    • 20
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    Immunofluorescence (IF), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-Complement C4d (C4D204), Purified, BSA-free
    产品特性
    This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    纯化方法
    Purified
    免疫原
    Recombinant human Complement 4d protein
    克隆位点
    C4D204
    亚型
    IgG1 kappa
  • 应用备注
    Immunohistology formalin-fixed 1:200-1:400
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 1:50-1:100
    • ELISA For coating use Ab at 1-2 μg/mL, order Ab without BSA
    • Optimal dilution for a specific application should be determined by user
    说明

    Rejected Renal Transplant Tissue

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS (no BSA, no azide)
    储存液
    Azide free
  • 抗原
    Complement C4d (C4d) (Complement Component C4d (C4d))
    别名
    Complement C4d (C4d 产品)
    分子量
    192 kDa (predicted)
    基因ID
    720, 721
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