ANO1 抗体 (CF®647)
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- 抗原 See all ANO1 抗体
- ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ANO1 antibody is conjugated to CF®647
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-DOG-1 (DG1/447), CF647 Conjugate
- 产品特性
- Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human DOG-1 protein
- 克隆位点
- DG1-447
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ANO1 Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells
- Optimal dilution for a specific application should be determined by user
- 说明
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Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
- 别名
- DOG-1 (ANO1 产品)
- 别名
- DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody
- 背景
- Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1,TAOS2, ORAOV2, TMEM16A
- 分子量
- ~114 kDa
- 基因ID
- 55107, 503074
- UniProt
- Q5XXA6
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