KRT15 抗体 (C-Term)
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- 抗原 See all KRT15 抗体
- KRT15 (Keratin 15 (KRT15))
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抗原表位
- C-Term
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适用
- 人, 大鼠, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This KRT15 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Mouse Monoclonal anti-Cytokeratin 15 (LHK15), Purified, with BSA
- 产品特性
- Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15 (exact sequence is proprietary)
- 克隆位点
- LHK15
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product KRT15 Primary Antibody
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- 应用备注
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Immunohistology (frozen or formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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A431 cells. Skin or Colon
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- KRT15 (Keratin 15 (KRT15))
- 别名
- Cytokeratin 15 (KRT15 产品)
- 别名
- xlk2 antibody, CK15 antibody, K15 antibody, K1CO antibody, AI528832 antibody, Krt1-15 antibody, Ka15 antibody, wu:fa91c06 antibody, wu:fa95d04 antibody, zgc:55334 antibody, keratin 15, type I L homeolog antibody, keratin 15, type I antibody, keratin 15 antibody, krt15.L antibody, krt15 antibody, KRT15 antibody, Krt15 antibody
- 背景
- CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15
- 分子量
- 52 kDa
- 基因ID
- 3866, 654570
- UniProt
- P19012
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