Cytochrome C 抗体 (CF®640R)
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- 抗原 See all Cytochrome C (CYCS) 抗体
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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适用
- 人, 大鼠, 小鼠, 马, 犬, Pigeon, Drosophila melanogaster, Frog
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytochrome C antibody is conjugated to CF®640R
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Cytochrome-C (CTC05), CF640R Conjugate
- 产品特性
- Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant cytochrome c protein
- 克隆位点
- CTC05
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CYCS Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
- 别名
- Cytochrome-C (CYCS 产品)
- 别名
- CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody
- 背景
- CYC, CYCS, HCS, THC4
- 分子量
- 15 kDa
- 基因ID
- 54205, 437060
- UniProt
- P99999
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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