Nucleolin 抗体
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- 抗原 See all Nucleolin (NCL) 抗体
- Nucleolin (NCL)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nucleolin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)
- 原理
- Mouse Monoclonal anti-Nucleolin (NCL/902), Purified, BSA-free
- 产品特性
- This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human NCL protein
- 克隆位点
- NCL-902
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NCL Primary Antibody
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Gerbil, Cat WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Gerbil, Cat WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) (N-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 兔, 马, Saccharomyces cerevisiae, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) (C-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 130CT43-8-5 unconjugated
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- 应用备注
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Does not react with mouse, rat or cow, others not known
- Immunohistology (formalin)
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin s lymphoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- Nucleolin (NCL)
- 别名
- Nucleolin (NCL 产品)
- 别名
- C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
- 背景
- NCL, Nucl, Nucleolin, Protein C23
- 分子量
- 76 kDa
- 基因ID
- 4691, 79110
- UniProt
- P19338
- 途径
- Ribonucleoprotein Complex Subunit Organization
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