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KRT1 抗体

KRT1 适用: 人, 大鼠, 小鼠, 兔, 小鸡, Cow, 猴, 犬 WB, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal AE-3 unconjugated
产品编号 ABIN6162000
发货至: 中国
  • 抗原 See all KRT1 抗体
    KRT1 (Cytokeratin HMW (KRT1))
    适用
    • 27
    • 27
    • 18
    • 9
    • 9
    • 9
    • 9
    • 8
    • 1
    人, 大鼠, 小鼠, 兔, 小鸡, Cow, 猴, 犬
    宿主
    • 27
    小鼠
    克隆类型
    • 27
    单克隆
    标记
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This KRT1 antibody is un-conjugated
    应用范围
    • 26
    • 26
    • 17
    • 15
    • 9
    • 9
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), Purified, with BSA
    产品特性
    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    纯化方法
    Purified
    免疫原
    Human epidermal keratin
    克隆位点
    AE-3
    亚型
    IgG1 kappa
    Top Product
    Discover our top product KRT1 Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    说明

    Epithelial cells, Skin or Adenocarcinomas

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    200 μg/mL
    缓冲液
    PBS/0.05 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    KRT1 (Cytokeratin HMW (KRT1))
    别名
    Cytokeratin, HMW (KRT1 产品)
    别名
    CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, keratin 1 antibody, KRT1 antibody
    背景
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    分子量
    52-67 kDa
    基因ID
    51350, 654392
    UniProt
    Q01546
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