Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
特异性
This antibody will detect recombinant Human soluble CEACAM-1. It shows cross-reactivity with several other CEACAM familiy members. There is no cross-reactivity with Human CEACAM-8.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Protein A Affinity Chromatography
免疫原
Highly pure recombinant Human soluble CEACAM-1 (N-term)
Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1, also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57 % aa identical to human CEACAM1, in the V-type Ig-like domain, they are 58 % and 56 % aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1