Melanoma Marker 抗体 (CF®647)
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- 抗原 See all Melanoma Marker products
- Melanoma Marker
- 适用
- 人, 犬, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Melanoma Marker antibody is conjugated to CF®647
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-Melanoma Marker (PNL2), CF647 Conjugate
- 产品特性
- Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Melanocyte antigen
- 克隆位点
- PNL2
- 亚型
- IgG1
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anti-Melanoma Marker antibody
适用: 人 IHC (p) 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Melanoma Marker antibody适用: 人 IHC, IF 宿主: 小鼠 Monoclonal KBA-62 unconjugated
anti-Melanoma Marker antibody适用: 人, 犬, 小鼠 IHC, IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
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- 应用备注
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Melanoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Melanoma Marker
- Abstract
- Melanoma Marker 产品
- 背景
- Human Melanoma Associated Antigen, PNL2
- 分子量
- Multiple (160 kDa, 100 kDa and <10 kDa)
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