HLA-DP beta 抗体 (CF®594)
-
- 抗原 See all HLA-DP beta products
- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HLA-DP beta antibody is conjugated to CF®594
- 应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), CF594 Conjugate
- 产品特性
- This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
- 克隆位点
- BRA-FB6
- 亚型
- IgG2b kappa
-
-
- 应用备注
-
Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
-
Raji cells. Tonsil or lymph node
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
-
- 抗原
- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
- 别名
- MHC-II (HLA-DP) (beta chain) (HLA-DP beta 产品)
- 分子量
- 33-35 kDa
- 基因ID
- 3115, 347270
- UniProt
- P04440
-