HLA-Aw32-&-HLA-A25 (MHC-I) 抗体 (CF®647)
适用: 人
FACS, IF, IHC (f)
宿主: 小鼠
Monoclonal
CATA-1
CF®647
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- 抗原
- HLA-Aw32-&-HLA-A25 (MHC-I)
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- CF®647
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-HLA-Aw32-&-HLA-A25 (MHC-I) (CATA-1), CF647 Conjugate
- 产品特性
- This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
- 克隆位点
- CATA-1
- 亚型
- IgG2a kappa
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anti-HLA-Aw32-&-HLA-A25 (MHC-I) antibody
适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CATA-1 unconjugated
anti-HLA-Aw32-&-HLA-A25 (MHC-I) antibody适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CATA-1 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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THP-1 and Jurkat cells. Tonsil or lymph node.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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