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CD44 Standard 抗体 (Biotin)

CD44s 适用: 人, Non-Human Primate FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal HCAM-918 Biotin
产品编号 ABIN6151582
发货至: 中国
  • 抗原 See all CD44 Standard (CD44s) 抗体
    CD44 Standard (CD44s)
    适用
    • 30
    • 12
    • 9
    • 9
    • 8
    • 6
    人, Non-Human Primate
    宿主
    • 35
    • 1
    小鼠
    克隆类型
    • 36
    单克隆
    标记
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD44 Standard antibody is conjugated to Biotin
    应用范围
    • 36
    • 30
    • 29
    • 10
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-CD44 Standard (HCAM/918), Biotin Conjugate
    产品特性
    This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Recombinant human HCAM protein
    克隆位点
    HCAM-918
    亚型
    IgG2a kappa
    Top Product
    Discover our top product CD44s Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    DU145 cells. Tonsil, lymph node or breast carcinomas.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    CD44 Standard (CD44s)
    别名
    CD44 Standard (CD44s 产品)
    分子量
    80-95 kDa
    基因ID
    960, 502328
    UniProt
    P16070
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