Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
Detects an ~37 kDa protein, corresponding to the apparent molecular mass of the Caspase-7 on SDS-PAGE Immunoblots, in samples from Human, Mouse, Rat, Monkey, Dog, Sheep, Rabbit, Pig and Hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kDa) and the fully processed Caspase-7 (p19) in apoptotic cell extracts.
交叉反应 (详细)
Species reactivity (expected):Canine, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep. Species reactivity (tested):Human.
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis, initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.Synonyms: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3