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CD28 抗体

Name: CD28 抗体
Brand: antibodies-online
SKU: ABIN611634
Availability: OutOfStock

CD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated

Flow cytometry surface staining pattern of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/mL) GAM APC. (CD28 抗体)

FACS

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/mL) GAM APC. (CD28 抗体)

FACS

2 images

(7 references)

产品编号 ABIN611634

发货至: 中国

Datasheet as PDF

抗原 See all CD28 抗体

CD28
适用
169

119

54

34

22

16

12

12

7

7

5

5

1

1

1

1
大鼠
宿主
126

77

28

16

8

3

2
小鼠
Compatible Secondaries
克隆类型
182

72

3
单克隆
标记
88

31

28

21

14

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD28 antibody is un-conjugated
应用范围
197

90

52

47

38

32

28

26

19

17

13

7

6

6

4

4

4

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP)

特异性

The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

交叉反应（详细）

Rat

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Mouse A20J B lymphoma cells transfected with rat CD28

克隆位点

JJ319

亚型

IgG1 kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal JJ319 PE

应用备注

Flow cytometry: Recommended dilution: 2-5 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Kerstan, Armbruster, Leverkus, Hünig: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).

van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, (2004) (PubMed).

Lühder, Huang, Dennehy, Guntermann, Müller, Winkler, Kerkau, Ikemizu, Davis, Hanke, Hünig: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, (2003) (PubMed).

Laskowski, Pratschke, Wilhelm, Dong, Beato, Taal, Gasser, Hancock, Sayegh, Tilney: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, (2002) (PubMed).

Haspot, Villemain, Laflamme, Coulon, Olive, Tiollier, Soulillou, Vanhove: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, (2002) (PubMed).

Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, (2001) (PubMed).

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
抗原

CD28

别名

CD28 (CD28 产品)

别名

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

背景

CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,Tp44

基因ID

25660

UniProt

P31042

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response