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CD8B 抗体

Name: CD8B 抗体
Brand: antibodies-online
SKU: ABIN609653
Availability: OutOfStock

CD8B 适用: 大鼠 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated

Flow cytometry surface staining pattern of rat splenocytes suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 μg/mL) GAM PE. (CD8B 抗体)

FACS

Surface staining of rat splenocytes using anti-CD8b (341) purified, GAM-APC. (CD8B 抗体)

FACS

2 images

(9 references)

产品编号 ABIN609653

发货至: 中国

Datasheet as PDF

抗原 See all CD8B 抗体

CD8B (CD8b Molecule (CD8B))
适用
76

24

20

17

4

4

4

4

3

3

2

2

2

2

1

1

1

1
大鼠
宿主
76

43

16
小鼠
Compatible Secondaries
克隆类型
78

56

1
单克隆
标记
58

17

17

12

7

7

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8B antibody is un-conjugated
应用范围
77

69

44

24

19

14

13

13

8

7

4

4

3

3

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

交叉反应（详细）

Rat

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

CD8 positive Wistar rat splenic T cell hybridomas

克隆位点

341

亚型

IgG1 kappa

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anti-CD8b Molecule (CD8B) antibody
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anti-CD8b Molecule (CD8B) (AA 51-150) antibody
CD8B 适用: 人 WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 FITC

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 1-243) antibody
CD8B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B 适用: 大鼠 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal H35-17-2 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Flow cytometry: Recommended dilution: 1.5 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Rossi, Del Principe, Rossi, Irno Consalvo, Luciano, Zucchetto, Bulian, Bomben, Dal Bo, Fangazio, Benedetti, Degan, Gaidano, Del Poeta, Gattei: "Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells." in: Journal of translational medicine, Vol. 8, pp. 23, (2010) (PubMed).

Vroblova, Vrbacky, Hrudkova, Jankovicova, Schmitzova, Maly, Krejsek, Smolej: "Significant change in ZAP-70 expression during the course of chronic lymphocytic leukemia." in: European journal of haematology, Vol. 84, Issue 6, pp. 513-7, (2010) (PubMed).

Mabarrack, Turner, Mayrhofer: "Recent thymic origin, differentiation, and turnover of regulatory T cells." in: Journal of leukocyte biology, Vol. 84, Issue 5, pp. 1287-97, (2008) (PubMed).

Nohara, Kulka, Déry, Wills, Hirji, Gilchrist, Befus: "Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5935-9, (2001) (PubMed).

Ellerman, Like: "Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat." in: Diabetes, Vol. 48, Issue 5, pp. 975-82, (1999) (PubMed).

Lin, Hirji, Nohara, Stenton, Gilchrist, Befus: "Mast cells express novel CD8 molecules that selectively modulate mediator secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6265-72, (1998) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." in: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).
抗原

CD8B (CD8b Molecule (CD8B))

别名

CD8b (CD8B 产品)

别名

Cd8b antibody, Ly-3 antibody, Ly-C antibody, Lyt-3 antibody, CD8B1 antibody, LEU2 antibody, LY3 antibody, LYT3 antibody, P37 antibody, Cd8b1 antibody, CD8b molecule antibody, CD8 antigen, beta chain 1 antibody, Cd8b antibody, CD8B antibody, Cd8b1 antibody

背景

CD8b molecule,The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

基因ID

926

UniProt

P10966

途径

TCR Signaling