V5 Epitope Tag 抗体 (Biotin)
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- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This V5 Epitope Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
- 序列
- GKPIPNPLLG LDST
- 特异性
- Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.
- 纯化方法
- Protein G purified
- 免疫原
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Paramyxovirus SV5, Simian-Virus 5.
Type of Immunogen: Virus - 亚型
- IgG2a
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- 应用备注
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Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:5000)
Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
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- 抗原
- V5 Epitope Tag
- 别名
- V5 Tag (V5 Epitope Tag 产品)
- 物质类
- Tag
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