Glutathione 抗体 (DyLight 488)
This anti-Glutathione antibody is a 小鼠 单克隆 antibody detecting Glutathione in FACS 和 IF. Suitable for .
Quick Overview for Glutathione 抗体 (DyLight 488) (ABIN577696)
抗原
See all Glutathione 抗体
Glutathione
适用
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宿主
小鼠
克隆类型
单克隆
标记
This Glutathione antibody is conjugated to DyLight 488
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
L4H
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品牌
- AbX™
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产品特性
- Designed for Immunofluorescence and FACS Analysis Supplied as 50 µg in PBS For use under Non-Reducing conditions
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纯化方法
- Purified
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免疫原
- Glutathione conjugated to Keyhole Limpet Hemocyanin
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亚型
- IgG2a
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应用备注
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1:5-1:50 IMMuNOFLuOresCeNCe Jurkat cells, permeabilized and incubated with our DyLight® 488 labeled Glutathione monoclo- nal, A001F-100UL, clone L4H, at a 1:25 dilution. 488 Filter unfiltered
Flow analysis of Jurkat cells processed for immunofluorescence as above. Unstained cells Stained at 1x Stained at 5x -
说明
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Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C
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储存方法
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- Glutathione
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物质类
- Chemical
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背景
- Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ DyLight® 488-labeled dye is similar to Alexa 488, Cy2 or FITC. It will measure GSH-protein complexes under non-reducing conditions.
抗原
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