IGF1 抗体 (Internal Region)
-
- 抗原 See all IGF1 抗体
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
-
抗原表位
- Internal Region
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This IGF1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- IGF1
- 序列
- RSVRAQRHTD
- 特异性
- This antibody is expected to recognize all reported isoforms (NP_001104753.1, NP_001104754.1, NP_001104755.1, NP_000609.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-RSVRAQRHTD
- 亚型
- IgG
- Top Product
- Discover our top product IGF1 Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 15 kDa band observed in Human Uterus lysates (calculated MW of 15.2 kDa according to NP_001104754.1). Recommended concentration 0.5-2 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
-
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- 别名
- IGF1 (IGF1 产品)
- 别名
- IGF-I antibody, IGF1A antibody, IGFI antibody, C730016P09Rik antibody, Igf-1 antibody, Igf-I antibody, Npt2B antibody, IGF-1 antibody, IGFIA antibody, IGF-IB antibody, igf1 antibody, IGF-1L antibody, IGF-1a antibody, IGF1 antibody, igf-1 antibody, igf1-A antibody, igf1.S antibody, xigf1 antibody, insulin like growth factor 1 antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I antibody, insulin like growth factor 1 L homeolog antibody, IGF1 antibody, Igf1 antibody, LOC100136741 antibody, igf1 antibody, LOC678666 antibody, igf1.L antibody, LOC104911603 antibody
- 背景
- IGF1, insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1
- 基因ID
- 3479, 16000, 24482
- NCBI登录号
- NP_001104753, NP_001104754, NP_001104755, NP_000609
- 途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
-