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Dystroglycan 抗体 (Internal Region)

Verified DAG1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN571179
发货至: 中国
  • 抗原 See all Dystroglycan (DAG1) 抗体
    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
    抗原表位
    • 15
    • 9
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 27
    • 20
    • 15
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 41
    • 4
    • 2
    山羊
    克隆类型
    • 43
    • 4
    多克隆
    标记
    • 22
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Dystroglycan antibody is un-conjugated
    应用范围
    • 41
    • 19
    • 17
    • 13
    • 13
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    DAG1
    序列
    HVGKHEYFMH ATDK
    特异性
    This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    C-HVGKHEYFMHATDK
    亚型
    IgG
    Top Product
    Discover our top product DAG1 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
    别名
    DAG1 (DAG1 产品)
    背景
    DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1
    基因ID
    1605
    NCBI登录号
    NP_004384
    途径
    Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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