POU3F3 抗体 (Internal Region)
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- 抗原 See all POU3F3 抗体
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
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抗原表位
- Internal Region
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This POU3F3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- POU3F3 / BRN1 / OCT8
- 序列
- HMLSHAHQWV TAL
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri
Peptide ELISA: antibody detection limit dilution 1:4000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
- 别名
- POU3F3 (POU3F3 产品)
- 别名
- BRN1 antibody, OTF8 antibody, Brn1 antibody, RHS1 antibody, Brn-1 antibody, Otf8 antibody, Skin1 antibody, brn1 antibody, nrl-20 antibody, nrl20-A antibody, pou1 antibody, pou23 antibody, wu:fb92g06 antibody, zfpou1 antibody, zp23 antibody, zp23pou antibody, BRN-1.1 antibody, ZP12POU antibody, brn1.1 antibody, pou12 antibody, wu:fc33a11 antibody, zp12 antibody, POU class 3 homeobox 3 antibody, POU domain, class 3, transcription factor 3 antibody, POU class 3 homeobox 3 S homeolog antibody, POU class 3 homeobox 3b antibody, POU class 3 homeobox 3a antibody, POU3F3 antibody, Pou3f3 antibody, pou3f3.S antibody, pou3f3b antibody, pou3f3a antibody
- 背景
- POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8
- 基因ID
- 5455, 18993, 192109
- NCBI登录号
- NP_006227
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