AIM2 抗体 (C-Term)
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- 抗原 See all AIM2 抗体
- AIM2 (Absent in Melanoma 2 (AIM2))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This AIM2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- AIM2
- 序列
- GEKLQLTSGV HSTIK
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.
- 亚型
- IgG
- Top Product
- Discover our top product AIM2 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- AIM2 (Absent in Melanoma 2 (AIM2))
- 别名
- AIM2 (AIM2 产品)
- 别名
- AIM2 antibody, PYHIN4 antibody, Gm1313 antibody, Ifi210 antibody, absent in melanoma 2 antibody, AIM2 antibody, Aim2 antibody
- 背景
- AIM2, absent in melanoma 2, PYHIN4
- 分子量
- 39.0kDa
- 基因ID
- 9447
- NCBI登录号
- NP_004824
- 途径
- Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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