Tppp 抗体 (Internal Region)
-
- 抗原 See all Tppp 抗体
- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
-
抗原表位
- Internal Region
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This Tppp antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- TPPP/p25
- 序列
- DPSKDRAAKR LSLES
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- C-DPSKDRAAKRLSLES
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
-
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
- 别名
- TPPP (Tppp 产品)
- 别名
- TPPP/p25 antibody, TPPP1 antibody, p24 antibody, p25 antibody, p25alpha antibody, 2900041A09Rik antibody, AI849835 antibody, RGD1310121 antibody, tubulin polymerization promoting protein antibody, TPPP antibody, Tppp antibody
- 背景
- TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
- 基因ID
- 11076, 72948, 361466
- NCBI登录号
- NP_008961
-