PRDM1 抗体 (Internal Region)
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- 抗原 See all PRDM1 抗体
- PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PRDM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- PRDM1 / MEL1
- 序列
- DISDNADRLE DVED
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_001189.2, NP_878911.1).
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2, NP_878911.1.
- 亚型
- IgG
- Top Product
- Discover our top product PRDM1 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.
Western Blot: Approx 90 kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100 kDa in lysates of cell line K562. An additional faint band of approx 70 kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8 kDa accor
Peptide ELISA: antibody detection limit dilution 1:1000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
- 别名
- PRDM1 (PRDM1 产品)
- 背景
- PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene posit
- 基因ID
- 639, 12142, 309871
- NCBI登录号
- NP_001189, NP_878911
- 途径
- Regulation of Muscle Cell Differentiation
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