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SATB1 抗体 (Internal Region)

Verified SATB1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN571004
发货至: 中国
  • 抗原 See all SATB1 抗体
    SATB1 (SATB Homeobox 1 (SATB1))
    抗原表位
    • 16
    • 9
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 62
    • 47
    • 28
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    宿主
    • 55
    • 7
    山羊
    克隆类型
    • 47
    • 15
    多克隆
    标记
    • 32
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SATB1 antibody is un-conjugated
    应用范围
    • 28
    • 21
    • 14
    • 14
    • 11
    • 11
    • 10
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    SATB1
    序列
    ESDEENRQKT RPRT
    交叉反应
    Cow, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.
    亚型
    IgG
    Top Product
    Discover our top product SATB1 Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5 μg/mL.

    Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0 kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 h

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    SATB1 (SATB Homeobox 1 (SATB1))
    别名
    SATB1 (SATB1 产品)
    别名
    2610306G12Rik antibody, AW413156 antibody, SATB1 antibody, sb:cb955 antibody, si:ch211-195k18.2 antibody, SATB homeobox 1 antibody, special AT-rich sequence binding protein 1 antibody, SATB homeobox 1a antibody, SATB1 antibody, Satb1 antibody, satb1 antibody, satb1a antibody
    背景
    SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)
    基因ID
    6304, 20230, 316164
    NCBI登录号
    NP_002962
    途径
    Caspase Cascade in Apoptosis, Activated T Cell Proliferation
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