IL-18 抗体 (Internal Region)
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- 抗原 See all IL-18 (IL18) 抗体
- IL-18 (IL18) (Interleukin 18 (IL18))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IL-18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- IL18
- 序列
- NPPDNIKDTK SDI
- 特异性
- This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.
- 亚型
- IgG
- Top Product
- Discover our top product IL18 Primary Antibody
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- 应用备注
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Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- IL-18 (IL18) (Interleukin 18 (IL18))
- 别名
- IL18 (IL18 产品)
- 别名
- Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody
- 背景
- IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18
- 基因ID
- 3606, 29197
- NCBI登录号
- NP_001553, NP_001230140
- 途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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