IKZF4 抗体 (Internal Region)
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- 抗原 See all IKZF4 抗体
- IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IKZF4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- IKZF4
- 序列
- RPTFIDRLAN SLTKR
- 特异性
- This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1
- 交叉反应
- Cow, 犬, 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.
- 亚型
- IgG
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- 应用备注
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Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0
Peptide ELISA: antibody detection limit dilution 1:2000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))
- 别名
- IKZF4 (IKZF4 产品)
- 别名
- A630026H08Rik antibody, Eos antibody, Zfpn1a4 antibody, Znfn1a4 antibody, EOS antibody, ZNFN1A4 antibody, IKAROS family zinc finger 4 antibody, Ikzf4 antibody, IKZF4 antibody
- 背景
- IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos
- 基因ID
- 64375, 22781
- NCBI登录号
- NP_071910
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