PDK1 抗体 (AA 107-336)
This anti-PDK1 antibody is a 兔 多克隆 antibody detecting PDK1 in WB, ELISA 和 IHC (p). Suitable for 人 和 大鼠.
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Quick Overview for PDK1 抗体 (AA 107-336) (ABIN5708184)
抗原
See all PDK1 抗体
PDK1
(Pyruvate Dehydrogenase Kinase 1 (PDK1))
适用
人, 大鼠
宿主
兔
克隆类型
多克隆
标记
This PDK1 antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- AA 107-336
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纯化方法
- Antigen affinity purified
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免疫原
- A recombinant human protein corresponding to amino acids V107-Y336 was used as the immunogen for the PDK1 antibody.
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亚型
- IgG
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anti-Pyruvate Dehydrogenase Kinase 1 (PDK1) antibody
PDK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase Kinase 1 (PDK1) (AA 29-436), (Isoform 1) antibody (APC)PDK1 适用: 人 IHC, FACS, ICC, IF 宿主: 兔 Polyclonal APC
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应用备注
- Optimal dilution of the PDK1 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL, IHC (FFPE): 1-2 μg/mL, Direct ELISA: 0.1-0.5 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the PDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
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别名
- PDK1 / Pyruvate Dehydrogenase Kinase 1
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背景
- PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
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UniProt
- Q15118
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途径
- PI3K-Akt Signaling, RTK signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect
抗原
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