Spartin (SPART) (Internal Region) 抗体
Verified
SPART
适用: 人
ELISA, IF, WB
宿主: 山羊
Polyclonal
unconjugated
-
- 抗原
- Spartin (SPART)
- 抗原表位
- Internal Region
- 适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- ELISA, Immunofluorescence (IF), Western Blotting (WB)
- 原理
- SPARTIN
- 序列
- EASGTDVKQL DQGNK
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EASGTDVKQLDQGNK, from the internal region of the protein sequence according to NP_055902.1.
- 亚型
- IgG
-
-
- 应用备注
-
Western Blot: Approx 70 kDa band observed in lysates of cell line HeLa (calculated MW of 72.8 kDa according to NP_055902.1). Recommended concentration: 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
-
Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- 抗原
- Spartin (SPART)
- 别名
- SPART
- 背景
- SPG20, spastic paraplegia 20 (Troyer syndrome), KIAA0610, SPARTIN, TAHCCP1, OTTHUMP00000018254, spastic paraplegia 20, spastic paraplegia 20, spartin (Troyer syndrome), trans-activated by hepatitis C virus core protein 1
- 基因ID
- 23111
- NCBI登录号
- NP_055902
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