E2F8 抗体 (Internal Region)
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- 抗原 See all E2F8 抗体
- E2F8 (E2F Transcription Factor 8 (E2F8))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This E2F8 antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- E2F8
- 序列
- KSIESDRRKI NSAPS
- 交叉反应
- Cow, 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.
- 亚型
- IgG
- Top Product
- Discover our top product E2F8 Primary Antibody
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- 应用备注
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Western Blot: Preliminary testing showed a band at approx 80 kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3 μg/mL antibody staining. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- E2F8 (E2F Transcription Factor 8 (E2F8))
- 别名
- E2F8 (E2F8 产品)
- 别名
- MGC130750 antibody, E2F8 antibody, E2F-8 antibody, RGD1308091 antibody, 4432406C08Rik antibody, AA410048 antibody, E2F transcription factor 8 antibody, E2F transcription factor 8 L homeolog antibody, E2F8 antibody, e2f8.L antibody, e2f8 antibody, E2f8 antibody
- 背景
- E2F8, E2F transcription factor 8, FLJ23311, E2F family member 8
- 基因ID
- 79733, 108961
- NCBI登录号
- NP_078956
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