Fluorescein 抗体
(2 references)
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- 抗原
- Fluorescein
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Supplier Product No.
- 200-301-096l
- Supplier
- Rockland
- 原理
- Fluorescein (FITC) Monoclonal Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.
- 产品特性
- Synonyms: mouse Anti-FITC antibody, mouse anti-fluorescein antibody
- 纯化方法
- Fluorescein Antibody was prepared from mouse ascites by Protein A chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Fluorescein conjugated to Mouse IgG
Immunogen Type: Other
- 克隆位点
- 8B9-C6-D3
- 亚型
- IgG2a kappa
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry Dilution: 1-2 μg/mL
Immunohistochemistry Dilution: 1:250 - 1:1,500
Application Note: Anti-Fluorescein Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:30,000
Other: in situ hybridization
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
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- 抗原
- Fluorescein
- 物质类
- Chemical
- 背景
- Background: Anti-Fluorescein is designed for immunofluorescence microscopy, in situ hybridization, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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