(1 reference)-
- 抗原
- Ngp (NGP) (Neutrophilic Granule Protein (NGP))
- 适用
- 小鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Fluorescence Microscopy (FM), Western Blotting (WB), Immunofluorescence (IF)
- 原理
- Ngp Antibody
- 交叉反应 （详细）
- Anti-NGP is directed against full-length mouse NGP.
- 产品特性
- Synonyms: rabbit anti-NGP antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
- 纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Affinity purified Anti-NGP antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse NGP protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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- 应用备注
-
Application Note: This affinity purified antibody has been tested for use in IF, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to NGP by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:200
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
-
: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
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: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
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- 抗原
- Ngp (NGP) (Neutrophilic Granule Protein (NGP))
- 别名
- Ngp
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.
- 基因ID
- 18054
- NCBI登录号
- NP_032720
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