Splicing Factor 1 抗体 (AA 160-266)
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- 抗原 See all Splicing Factor 1 (SF1) 抗体
- Splicing Factor 1 (SF1)
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抗原表位
- AA 160-266
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Splicing Factor 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 品牌
- Picoband™
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
- Rabbit IgG polyclonal antibody for splicing factor 1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- 免疫原
- E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
- Top Product
- Discover our top product SF1 Primary Antibody
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- 应用备注
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Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- Splicing Factor 1 (SF1)
- 别名
- SF1 (SF1 产品)
- 别名
- BBP antibody, D11S636 antibody, MBBP antibody, ZCCHC25 antibody, ZFM1 antibody, ZNF162 antibody, BB094781 antibody, CW17R antibody, MZFM antibody, WBP4 antibody, Zfp162 antibody, CG5836 antibody, Dmel\\CG5836 antibody, cg5836 antibody, dSF1 antibody, p70 antibody, SF1 antibody, sf1 antibody, wu:fc09f06 antibody, znf162 antibody, MWD22.25 antibody, MWD22_25 antibody, SF-1 antibody, splicing factor 1 antibody, Splicing factor 1 antibody, splicing factor-like protein antibody, nuclear receptor subfamily 5 group A member 1 antibody, splicing factor 1 S homeolog antibody, SF1 antibody, Sf1 antibody, sf1 antibody, AT5G51300 antibody, NR5A1 antibody, sf1.S antibody
- 背景
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Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162
Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
- UniProt
- Q15637
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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