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TFF3 抗体 (AA 23-81)

Name: TFF3 抗体 (AA 23-81)
SKU: ABIN5692886
Availability: OutOfStock
Rating: 5 (1 reviews)

TFF3 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 23-81) antibody (ABIN5692886) (TFF3 抗体 (AA 23-81))

Western blot analysis of TFF3 using anti-TFF3 antibody . (TFF3 抗体 (AA 23-81))

IHC analysis of TFF3 using anti-TFF3 antibody . (TFF3 抗体 (AA 23-81))

IHC

3 images

(1 validation)

产品编号 ABIN5692886

发货至: 中国

Datasheet as PDF

抗原 See all TFF3 抗体

TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))
抗原表位
16

8

7

5

3

2

2

1

1

1

1
AA 23-81
适用
53

24

23

6

5

1
小鼠, 大鼠
宿主
62

12

1
兔
Compatible Secondaries
克隆类型
62

13
多克隆
标记
27

17

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This TFF3 antibody is un-conjugated
应用范围
52

25

23

14

13

13

9

8

8

7

4

3

3

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

品牌

Picoband™

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for TFF3 detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.

免疫原

E. coli-derived mouse TFF3 recombinant protein (Position: A23-F81).

Top Product

Discover our top product TFF3 Primary Antibody
anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 51-80) antibody
TFF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 23-81) antibody
TFF3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 22-80) antibody
TFF3 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 15-73) antibody
TFF3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3D9 unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 23-81) antibody
TFF3 适用: 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 15-74) antibody
TFF3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3D9 unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 23-81) antibody
TFF3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) (AA 22-80) antibody
TFF3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) antibody
TFF3 适用: 人 ELISA, IHC, IF 宿主: 小鼠 Monoclonal 1D3G1 unconjugated

anti-Trefoil Factor 3 (Intestinal) (TFF3) antibody
TFF3 适用: 人 IHC 宿主: 兔 Monoclonal unconjugated

应用备注

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

限制

仅限研究用
生效 #103596 (Western Blotting)

by

Laboratory for neurodegenerative disease research, Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia

No.

#103596

日期

2019.02.18

抗原

Tff3

Lot Number

0011712Da513858

Method validated

Western Blotting

Positive Control

colon from WT C57BL/6 mice

Negative Control

small intestine from Tff3 deficient mice

Notes

ABIN5692886 specifically recognizes Tff3 in colon and small intestine tissue of wild type C57BL/6 mice.

Validation Images

ABIN5692886 specifically recognizes Tff3 in colon and small intestine tissue of wild type C57BL/6 mice.
Full Methods
Primary Antibody

ABIN5692886

Secondary Antibody

goat anti-rabbit IgG-HRP antibody (Bio-Rad, 170-6515, L005679 A)

Full Protocol

Isolate colon and small intestine tissue of wild type C57BL/6 mice and small intestine of Tff3 deficient mice using RIPA buffer (50mM TRIS HCL, pH8, 150mM NaCl, 1mM EDTA, 1% NP40 (Sigma-Aldrich, I-3021), 1% sodium deoxycholate (Sigma-Aldrich, D6750-25g), 0.1% SDS) containing PhosSTOP phosphatase inhibitor (Roche, 04906845001, lot 27423300) and cOmplete Mini protease inhibitor (Roche, lot 25765800).
Determine total protein concentration using Pierce BCA protein assay kit (ThermoFisher Scientific, 23227, lot Tf268093).
Denature 30µg total protein for 5min at 95°C in 6µl 5x Laemmli SDS sample buffer, 3µl 3M DTT and 1.5ul β-mercaptoethanol and Milli-Q H₂0 in 30µl total volume per sample.
Separate proteins on a freshly cast reducing 15% SDS-PAGE gel at 100V for the stacking gel and 150V for the running gel for 2-3 hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell chamber (Bio-Rad).
Transfer proteins onto a nitrocellulose membrane using Trans-Blot Transfer Medium (Bio-Rad, 162-0112, lot BR 8158009) on a Trans-Blot Turbo Transfer System (Bio-Rad, 1704150) at 150mA for 90min.
Stain membrane using Ponceau S to verify protein transfer.
Wash membrane with TBS.
Block the membrane with 1x TBS, 5% non-fat Milk, 0.1% Tween) for 1h at RT.
Incubate membrane with primary
polyclonal rabbit anti-TFF3 antibody (antibodies-online, ABIN5692886, lot 0011712Da513858) diluted 1:1000 in 1x TBS, 5% BSA, 0.1% Tween ON at 4°C.
polyclonal rabbit anti-TFF3 antibody (in-house) diluted in 1x TBS, 5% BSA, 0.1% Tween ON at 4°C.
Wash membrane 3x for 10min with TBST (1x TBS, 0.1% Tween).
Incubate membrane with secondary goat anti-rabbit IgG-HRP antibody (Bio-Rad, 170-6515, L005679 A) diluted 1: 5000 in TBST for 1.5h at RT.
Wash membrane 3x for 10min with TBST.
Reveal proteins bands using Pierce ECL Western Blotting Substrate (ThermoFisher Scientific, 32106, lot NJ175924) on an Imager Alliance 4.7 western blot imaging system (Uvitec).

Experimental Notes

ABIN5692886 reveals a protein of the expected molecular weight of the antigen in mice tissue. The protein bands is only visible in the positive but not the negative control. The same protein band was also detected by an in-house anti-TFF3 antibody hat was used as a reference.
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
抗原

TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

别名

Tff3 (TFF3 产品)

别名

TFF3 antibody, ITF antibody, P1B antibody, TFI antibody, mITF antibody, trefoil factor 3 antibody, trefoil factor 3, intestinal antibody, TFF3 antibody, Tff3 antibody

背景

Synonyms: Trefoil factor 3, Intestinal trefoil factor, mITF, Tff3, Itf
Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed abundantly in goblet cells of intestine and colon, and at low levels in stomach. No expression in brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus.
Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

UniProt

Q62395