Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to carboxypeptidase A from Bovine pancreas.
交叉反应 (详细)
Cross-reactivities against enzymes of other sources may occur but have not been determined.
产品特性
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
免疫原
Carboxypeptidase A isolated and purified from bovine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
CPA
适用: Cow
WB, ELISA, DB
宿主: 兔
Polyclonal
unconjugated
应用备注
Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/200 and 1/10,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
缓冲液
Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
储存液
Without preservative
储存条件
RT,4 °C,-20 °C
储存方法
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.