Ccl12 抗体 (Biotin)
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- 抗原 See all Ccl12 抗体
- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ccl12 antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB)
- 纯化方法
- Anti-mMCP-5 specific antibody was purified by affinity chromatography and then biotinylated.
- 免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).
- Top Product
- Discover our top product Ccl12 Primary Antibody
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- 应用备注
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ELISA:
To detect mMCP-5 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-5.
Sandwich:
To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-5 (XP-5226) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.
Western Blot:
To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存条件
- -20 °C
- 储存方法
- MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
- 别名
- MCP-5 (Ccl12 产品)
- 背景
- MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
- 基因ID
- 20293
- UniProt
- Q62401
- 途径
- Cellular Response to Molecule of Bacterial Origin
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