KC 抗体 (Biotin)
适用: 小鼠
WB, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原
- KC
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Anti-mKC specific antibody was purified by affinity chromatography and then biotinylated.
- 免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mKC (murine KC).
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- 应用备注
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ELISA:
To detect mKC by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mKC.
Sandwich:
To detect mKC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine KC (XP-5207) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mKC.
Western Blot:
To detect mKC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mKC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存条件
- -20 °C
- 储存方法
- KC antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- KC
- 基因ID
- 14825
- UniProt
- P12850
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