ELISA: Sandwich: In a sandwich ELISA (assuming 100μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 500 pg/mL of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
-20 °C
储存方法
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.