Concanavalin A 抗体 (HRP)
ConA
适用: Jack Bean
ID, IHC (fro), IF
宿主: 山羊
Polyclonal
HRP
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- 抗原 See all Concanavalin A (ConA) 抗体
- Concanavalin A (ConA)
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适用
- Jack Bean
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Concanavalin A antibody is conjugated to HRP
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应用范围
- Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- 产品特性
- Molar Ratio: Peroxidase/IgG: ~ 1.3
- 纯化方法
- Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. Undesired traces of antibody activity are eliminated by Immunoaffinity chromatography
- 免疫原
- The lectin Concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product ConA Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore by adding 1 mL sterile di stilled water.
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 withouit preservatives.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Concanavalin A (ConA)
- 别名
- Concanavalin-A (ConA 产品)
- 背景
- Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean, Canavalia ensiformis. It is a member of the legume lectin family. It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. ConA is a plant mitogen, and is known for its ability to stimulate mouse T-cell subsets giving rise to four functionally distinct T cell populations, including precursors to suppressor T-cell, one subset of human suppressor T-cells as well is sensitive to ConA. ConA was the first lectin to be available on a commercial basis, and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells.Synonyms: Con-A, ConA, Concanavalin A
- 基因ID
- 3823
- UniProt
- P02866
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