Ficin 抗体
适用: Fig
IF, WB, DB, EIA, ID, IP, RIA
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all Ficin products
- Ficin
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适用
- Fig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Ficin antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- 免疫原
- Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 溶解方式
- Restore by adding 1.0 mL of sterile distilled water
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- 抗原
- Ficin
- Abstract
- Ficin 产品
- 背景
- Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
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