Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
特异性
IgG fraction of polyclonal rabbit antiserum to phosphomannose isomerase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatog
免疫原
Phosphomannose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure