Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
L-Lactic Dehydrogenase LDH-1(M4) isoenzyme, is isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Restore by adding 1.0 mL of sterile distilled water
浓度
10.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.