Glucuronidase beta 抗体 (Biotin)
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- 抗原 See all Glucuronidase beta (GUSB) 抗体
- Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
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适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Glucuronidase beta antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)
- 特异性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Molar Ratio: Biotin/IgG~ 6.2
- 纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- 免疫原
- Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product GUSB Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore by adding 1.0 mL of sterile distilled water.
- 浓度
- 10 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- 抗原
- Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))
- 别名
- beta-Glucuronidase (GUSB 产品)
- 别名
- GUSB antibody, beta-GUS antibody, gus antibody, BG antibody, MPS7 antibody, AI747421 antibody, Gur antibody, Gus antibody, Gus-r antibody, Gus-s antibody, Gus-t antibody, Gus-u antibody, Gut antibody, asd antibody, g antibody, Ac2-223 antibody, si:ch211-160e1.7 antibody, si:ct573103.7 antibody, glucuronidase beta antibody, beta-D-glucuronidase antibody, beta-glucuronidase antibody, beta glucuronidase antibody, glucuronidase, beta antibody, GUSB antibody, bglR antibody, SSO_RS14735 antibody, Glu antibody, Gusb antibody, gusb antibody
- 背景
- Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB
- 基因ID
- 946149
- NCBI登录号
- AP_002238
- UniProt
- P05804
- 途径
- Glycosaminoglycan Metabolic Process
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