Human and bovine procollagen type III (PIIIP) 100 % , human and bovine collagen type III very low at RIA dilution <1:100. Human and bovine collagen type I <0.1 % in RIA at 1:200 dilution.
纯化方法
Affinity purified
免疫原
Procollagen type III deprived of the C-terminal PIIICP and N-terminal PIIINP
IFA, IHC(P), ELISA, RIA , After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human or bovine skin and liver
限制
仅限研究用
状态
Lyophilized
浓度
approx. 1 mg/mL
缓冲液
phosphate buffered solution, no BSA and preservative added!