Cyclin E1 抗体
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- 抗原 See all Cyclin E1 (CCNE1) 抗体
- Cyclin E1 (CCNE1)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cyclin E1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody reacts with Cyclin E (50 kDa) on Western blotting. This monoclonal antibody may nonspecifically react with macrophages, endothelial cells and some stromal cells in Immunohistochemistry.
- 纯化方法
- Protein-A Agarose Chromatography of hybridoma supernatant.
- 免疫原
- Human recombinant full length His-Cyclin E.
- 克隆位点
- HE12
- 亚型
- IgG1
- Top Product
- Discover our top product CCNE1 Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- Cyclin E1 (CCNE1)
- 别名
- Cyclin E1 (CCNE1 产品)
- 背景
- Cyclin E, a positive regulator of the cell cycle, controls the transition of cells from G1 to S phase. Cyclin E forms a complex with Cdk2, the active kinase follows cyclin D/Cdk4 in progressively phosphorylating pRb, releasing it from members of the E2F family. E2F transcrIPPtion factors in turn activate a set of target genes that are essential for entry into S phase. Deregulation of the G1-S checkpoint contributes to uncontrolled cell division, a hallmark of cancer. Cyclin E overexpression and deregulation have been shown in several cancers including breast, colorectal, gastric and lungSynonyms: CCNE, CCNE1, Cyclin-E1
- 基因ID
- 898
- NCBI登录号
- NP_001229
- UniProt
- P24864
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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