Immunohistochemistry (IHC), Western Blotting (WB), ELISA
交叉反应 (详细)
human TGFalpha 100%. (Neutralizing antibody)We found no crossreactivty to other cytokines such as hTGFß, hEGF, hPDGf, hTNF. hEPO, hIGF, 1mg per ml. hIL-1, hIL-2, hIL-3, hIL-4, hIL-5, hIL-6, hIL-7, hIL-8, hIL-9, hIL-10, hIL-11,and hIL-12
纯化方法
Purified
内毒素水平
<0.1 EU per 1 µg of the antibody as determined by the LAL method.
免疫原
TGF alpha antibody was raised in goat using E. coli-derived recombinant human TGF-alpha as the immunogen.
ELISA: 2-8 µg/mL, IHC: 15 µg/mL, WB: 1 µg/mL Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with PBS buffer.
浓度
Lot specific
缓冲液
Supplied as a lyophilized powder.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
Transforming growth factor alpha (TGF- alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGF alpha stimulates neural cell proliferation in the adult injured brain.