Recombinant AOC3 抗体
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- 抗原 See all AOC3 抗体
- AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This AOC3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunoaffinity Chromatography (IAC), Inhibition (Inh)
- 原理
- Anti-VAP-1 [TK8-14], Rabbit IgG, kappa
- 特异性
- This antibody is specific for human VAP-1, a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. The recognized epitope is present both in dimeric and monomeric forms of VAP-1.
- 产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing specific pathogen-free BALB/c mice with affinity-purified VAP-1 obtained from tonsil stromal lysate.
- 克隆位点
- TK8-14
- 亚型
- IgG kappa
- Top Product
- Discover our top product AOC3 Primary Antibody
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anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (AA 27-190) antibody
AOC3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (AA 613-640) antibodyAOC3 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB22029 unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (AA 27-259) antibodyAOC3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (AA 1-763) antibodyAOC3 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (AA 351-450) antibodyAOC3 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 4B8 unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) (Internal Region) antibodyAOC3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) antibodyAOC3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) antibodyAOC3 适用: 人 FACS, IHC (fro), Func 宿主: 小鼠 Monoclonal 174-5 unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) antibodyAOC3 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) antibodyAOC3 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170- kDa VAP-1 and, under reducing conditions, with the monomeric 90- kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998, PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijärvi et al., 1998, PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry, it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration. The results showed a significant reduction in monocyte transmigration upon pretreatment, indicating the antibody's role as an inhibitor of VAP-1 function (Zimmermann et al., 2012, DOI: 10.1136/gutjnl-2012-302514b.126). This antibody was used in flow cytometry and immunocytochemistry to detect and characterize human hematopoietic stem cells based on VAP-1 expression. In flow cytometry, this antibody was utilized to stain fresh human bone marrow and cord blood (CB) cells. In an immunocytochemistry assay, it was used to stain FACS-sorted CD34+ CB cells for visualization under a microscope. Furthermore, TK8-14 was utilized in RNAseq analysis to investigate gene expression profiles of VAP-1+ and VAP-1- HSCs (Iftakhar-E-Khuda et al., 2021, PMID: 34719737).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))
- 别名
- VAP-1 (AOC3 产品)
- 背景
- Vascular adhesion protein 1, VAP1, Copper amine oxidase, HPAO, Membrane primary amine oxidase, Vascular adhesion protein 1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase 1, Semicarbazide-sensitive, AOC3, Amine oxidase, copper containing 3, amine oxidase, amine oxidase, Vascular adhesion protein 1, SSAO, copper-containing amine:oxy, EC:1.4.3.21
- UniProt
- Q16853
- 途径
- Feeding Behaviour
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