Recombinant Fluorescein 抗体
(1 reference)-
- 抗原
- Fluorescein
- 抗体类型
- Recombinant Antibody
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宿主
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Human
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- In vivo Studies (in vivo), Negative Control (NC)
- 原理
- Anti-Fluorescein [4-4-20 (enhanced)], Human IgG1, kappa
- 特异性
- This antibody is specific for fluorescein I.
- 交叉反应
- Various Species
- 产品特性
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Original Species of Ab: Human
Original Format of Ab: scFv
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).
- 克隆位点
- 4-4-20 (enhanced)
- 亚型
- IgG1 kappa
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999, PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.
- 说明
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This is a reformatted antibody made using the variable domain sequences of the original human scFv format to improve compatibility with existing reagents, assays, and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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: "Anti-PD1 'SHR-1210' aberrantly targets pro-angiogenic receptors and this polyspecificity can be ablated by paratope refinement." in: mAbs, Vol. 11, Issue 1, pp. 26-44, (2019) (PubMed).
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: "Anti-PD1 'SHR-1210' aberrantly targets pro-angiogenic receptors and this polyspecificity can be ablated by paratope refinement." in: mAbs, Vol. 11, Issue 1, pp. 26-44, (2019) (PubMed).
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- 抗原
- Fluorescein
- 物质类
- Chemical
- 背景
- FL, FlI, FITC, Fluorescein isothiocyanate, fluorescein isothiocyanate, isomer 1, Fluorescyl, Resorcinolphthalein, C.I. 45350, solvent yellow 94, D & C yellow no. 7, Angiofluor, Japan yellow 201, Soap yellow, 3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
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