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Calpastatin 抗体 (AA 275-310)

Name: Calpastatin 抗体 (AA 275-310)
SKU: ABIN5647087
Availability: OutOfStock

CAST 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

Western blot testing of human COLO320 cell lysate with Calpastatin antibody at 0. (Calpastatin 抗体 (AA 275-310))

Flow cytometry testing of human A431 cells with Calpastatin antibody at 1ug/10^6 cells (blocked with goat sera) (Calpastatin 抗体 (AA 275-310))

FACS

IHC testing of FFPE human intestinal cancer tissue with Calpastatin antibody at 1ug/ml. (Calpastatin 抗体 (AA 275-310))

IHC

Flow cytometry testing of human U-2 OS cells with Calpastatin antibody at 1ug/10^6 cells (blocked with goat sera) (Calpastatin 抗体 (AA 275-310))

FACS

Flow cytometry testing of human A549 cells with Calpastatin antibody at 1ug/10^6 cells (blocked with goat sera) (Calpastatin 抗体 (AA 275-310))

FACS

5 images

产品编号 ABIN5647087

发货至: 中国

Datasheet as PDF

抗原 See all Calpastatin (CAST) 抗体

Calpastatin (CAST)
抗原表位
15

7

3

2

1

1

1

1

1

1

1

1

1

1
AA 275-310
适用
51

21

5

2
人
宿主
41

12
兔
Compatible Secondaries
克隆类型
44

9
多克隆
标记
27

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Calpastatin antibody is un-conjugated
应用范围
38

15

13

13

7

7

7

4

3

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

纯化方法

Antigen affinity purified

免疫原

Amino acids 275-310 (QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR-human) were used as the immunogen for the Calpastatin antibody.

亚型

IgG

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CAST 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-667) antibody
CAST 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 582-669) antibody
CAST 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated

anti-Calpastatin (CAST) antibody
CAST 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 431-530) antibody
CAST 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-686) antibody
CAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-667) antibody
CAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Calpastatin (CAST) antibody (FITC)
CAST 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC

anti-Calpastatin (CAST) (AA 275-700) antibody
CAST 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal dilution of the Calpastatin antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells,Immunohistochemistry (FFPE): 1-2 μg/mL

限制

仅限研究用
缓冲液

0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

储存条件

-20 °C

储存方法

After reconstitution, the Calpastatin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
抗原

Calpastatin (CAST)

别名

Calpastatin (CAST 产品)

别名

BS-17 antibody, calp3 antibody, CAST antibody, sb:cb966 antibody, zgc:194249 antibody, cast antibody, DKFZp469E0716 antibody, calpastatin antibody, calpastatin S homeolog antibody, Calpastatin antibody, CAST antibody, Cast antibody, cast.S antibody, cast antibody, VDBG_09182 antibody, ical antibody

背景

Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt

P20810