Western Blotting (WB), Immunocytochemistry (ICC), ELISA
产品特性
This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
纯化方法
Purified
免疫原
A phospho-peptide corresponding to phospho-Histone H2AX (S139) was used as the immunogen for this recombinant Gamma H2AX antibody.
The stated application concentrations are suggested starting points. Titration of the recombinant Gamma H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL
限制
仅限研究用
缓冲液
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store the recombinant Gamma H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.