SOX10 抗体 (AA 115-269)
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- 抗原 See all SOX10 抗体
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
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抗原表位
- AA 115-269
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SOX10 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This mAb recognizes a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
- 纯化方法
- Purified
- 纯度
- Protein G affinity chromatography
- 免疫原
- Amino acids 115-269 of the human protein were used as the immunogen for the SOX10 antibody.
- 克隆位点
- SOX10-991
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product SOX10 Primary Antibody
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anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibody
SOX10 适用: 人, 小鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal SOX10-991 unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (Middle Region) antibodySOX10 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 147-252) antibodySOX10 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 2E7E8 unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 147-252) antibodySOX10 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 2E7B5 unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 231-259) antibodySOX10 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB21835 unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibodySOX10 适用: 人, 小鼠 IHC, StM 宿主: 兔 Monoclonal SOX10-2311R unconjugated Recombinant Antibody
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibodySOX10 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal rSOX10-1074 unconjugated Recombinant Antibody
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibodySOX10 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal SOX10-992 unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 200-300) antibodySOX10 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 231-259) antibodySOX10 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the SOX10 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the SOX10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
- 别名
- SOX10 (SOX10 产品)
- 别名
- DOM antibody, PCWH antibody, WS2E antibody, WS4 antibody, WS4C antibody, SOX-10 antibody, SOX10 antibody, dom antibody, ws4 antibody, Dom antibody, Sox21 antibody, SOX9 antibody, cls/sox10 antibody, zgc:100757 antibody, SRY-box 10 antibody, SRY box 10 antibody, SRY-box 10 L homeolog antibody, SRY (sex determining region Y)-box 10 antibody, SOX10 antibody, Sox10 antibody, sox10.L antibody, sox10 antibody
- 背景
- This mAb recognizes a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
- 途径
- Chromatin Binding
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